PTEN stands for Phosphatidylinositol-3, 4, and 5-trisphosphate, as well as 3-phosphatase and dual-specificity protein phosphatase and the antibody is a tumor suppressor, as well as a member of the PI3K/PTEN/Akt pathway. Defects of such antibodies have been implicated in a variety of human cancers, including thyroid, prostate, skin, breast, head, neck, brain, and endometrium. Up to 60 percent of all advanced prostate cancers show an abnormal PTEN gene expression or loss of the protein expression altogether.
This antibody has a clone called SP218, and the immunogen is the synthetic peptide that is derived from the c-terminus of the same protein. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 54kDa. It can also be used in a variety of applications.
For the IHC procedure, youll want to use paraffin-embedded or Formalin-fixed tissues and deparaffinized slides. When using the concentrated format, you should dilute the antibody using the ratio 1:200, though this is an approximation. To retrieve the antigen, youll need to boil a tissue section in a 10mM citrate buffer with a pH of 6.0 for 10 minutes, allowing it to cool for 20 minutes. Incubate the antigen for 10 minutes at room temperature. The positive control is the prostate Adenocarcinoma or prostate.
For Western Blotting, youll want to dilute the antibody using a ratio of 1:400, incubating for one hour at room temperature. The positive control is the HeLa Cell Lysate. Also, it is important to note that while a pre-diluted formulation is available, it should not be used for Western Blotting.
For Flow Cytometry, the positive control is the A431 Cell Line with cellular localization occurring in the Cytoplasm or nucleus.
The PTEN antibody can be used for researching a variety of cancers. Visit Spring BioScience now to learn more.